Efficacy of Benzylkonium and Clorhexidine against Clinical Isolates of Pseudomonas Sp. from Dhaka, Bangladesh

The opportunistic pathogen Pseudomonas aeruginosa has been recognized as a major cause of nosocomial infection due to resistance multiple classes antibiotics. This study reports presence carbapenem genes in clinical isolates from Dhaka, Bangladesh, well their some common disinfectants (benzylkonium chloride and chlorhexidine gluconate). Twenty three samples were collected two hospitals one diagnostic center different locations Dhaka city. Among these, 16 identified sp. growth on Cetrimide Agar, standard biochemical tests (catalase, oxidase triple sugar iron, urea, Sulphur Indole Motility medium, Lysine Iron Methyl Red-Voges Proskaeur nitrate, citrate) IasL/R-gene targeted PCR. Molecular characterization revealed the contained 2.3kb 2.5 kb plasmids. genome 6 blaGIM gene encoding metallo-beta-lactamase variant (German), but none resistant against imipenem group antibiotics (meropenem, gentamicin, cefixime, imipenem). Therefore are genetically potent resist belonging group, still sensitive culture. 3% Benzylkonium 0.5% gluconate slightly synergistic effect inhibiting representative Fractional Inhibitory Concentration Index value ranges between 0.22 0.5. Taken together, harboring potential virulent imipenem-resistance could be inhibited more effectively vitro if than suitable applied combinedly.